Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis

论文摘要

Rana kunyuensis is a species of brown frog that lives exclusively on Kunyu Mountain, Yantai, China. In the current study, a 279-bp cD NA sequence encoding a novel antimicrobial peptide（AMP）, designated as amurin-9 KY,was cloned from synthesized double-strand skin cD NA of R. kunyuensis. The amurin-9 KY precursor was composed of 62 amino acid（aa） residues, whereas the mature peptide was composed of 14 aa and contained two cysteines forming a C-terminal heptapeptide ring（Rana box domain） and an amidated C-terminus. These structural characters represent a novel amphibian AMP family. Although amurin-9 KY exhibited high similarity to the already identified amurin-9 AM from R. amurensis,little is known about the structures and activities of amurin-9 family AMPs so far. Therefore, amurin-9 KY and its three derivatives（amurin-9 KY1–3） were designed and synthesized. The structures and activities were examined to evaluate the influence of C-terminal amidation and the heptapeptide ring on the activities and structure of amurin-9 KY. Results indicated that C-terminal amidation was essential for antimicrobial activity, whereas both C-terminal amidation and the heptapeptide ring played roles in the low hemolytic activity. Circular dichroism（CD） spectra showed that the four peptides adopted anα-helical conformation in THF/H2 O（v/v 1:1） solution,but a random coil in aqueous solution. Elimination of the C-terminal heptapeptide ring generated two free cysteine residues with unpaired thiol groups,which greatly increased the concentration-dependent anti-oxidant activity. Scanning electron microscopy（SEM）was also performed to determine the possible bactericidal mechanisms.

论文目录

文章来源

类型: 期刊论文

作者: Fen Zhang,Zhi-Lai Guo,Yan Chen,Li Li,Hai-Ning Yu,Yi-Peng Wang

来源: Zoological Research 2019年03期

年度: 2019

分类: 基础科学,农业科技

专业: 生物学

单位: Department of Pharmaceutical Sciences, College of Pharmaceutical Sciences, Soochow University,School of Life Sciences, Guizhou Normal University,Department of Bioscience and Biotechnology, Dalian University of Technology

基金: supported by grants from the National Natural Science Foundation of China(31772455),Natural Science Foundation of Jiangsu Province(BK20160336and BK20171214),Natural Science Foundation of College in Jiangsu Province(16KJB350004),Suzhou Science and Technology Development Project(SYN201504 and SNG2017045)

分类号: Q51

页码: 198-204

总页数: 7

文件大小: 3693K

下载量: 13

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